rep wrote:I found this on a thread (http://pipeline.corante.com/archives/2013/03/05/taurxs_funding_is_odd.php) questioning the funding of TaoRx. Turns out the person names Liz is TaoRx's media person.
Methylene blue has been demonstrated to be a potent monoamine oxidase inhibitor (MAOI) and may cause potentially fatal serotonin toxicity (serotonin syndrome) when combined with serotonin reputake inhibitors (SRIs). (4) (See DRUG INTERACTIONS.) Serotonin toxicity is characterized by development of neuromuscular hyperactivity (tremor, clonus, myoclonus and hyperreflexia, and, in the advanced stage, pyramidal rigidity); autonomic hyperactivity (diaphoresis, fever, tachycardia, tachypnoea, and mydraisis); and altered mental status (agitation, excitement, and in the advanced stage, confusion). If methylene blue is judged to be indicated, SRIs must be ceased, prior to treatment/procedure/surgery.
Hi aaron43, thanks for posting your experiences with MB. I was referring to the idea that we shouldn't mix 60ug doses of MB with SSRIs. I think that 60ug is far to low a dose for its MAOI inhibition to present a danger when combined with serotonin-raising compounds, even though MB is a relatively potent MAOI. Ramsay et al. find that MB has an IC50 for MAO A of 164 nM. Ramsay's paper is in regards to the danger of combining MB with antidepressants, but they are talking about huge doses, like 100mg taken intravenously. That would be like taking a gram orally. As a back of the envelope calculation considering the blood level of MB produced by taking 60ug i.v., 60ug MB * (um MB/320 ug MB) / 5 litres blood = 37.5nM. This is well under the IC50 for the enzyme, so you would retain most of your MAO A activity. With oral dosing, you would have yet another factor of ten (-ish) margin of safety.
Bottom line: While MB is an MAO A inhibitor at high doses, 60ug isn't enough to get a dangerous MAO A inhibition. I don't doubt that you are feeling something from low dose MB. Have you tried it by itself, without the noots?
Posted 28 June 2011 - 02:00 PM
I love being a devil's advocate, but not today. Methylene Blue as an MAOI at the dose Aaron is taking is subtle to say the least. 60mcg of a REVERSIBLE MAOI is unlikely to cause any adverse side effects like a hypomanic state. The effects of Methylene Blue are primarily inhibiting enzymes related to aging, boosting mitochondria health back into a youthful state. This is not antidepressant medication, but an anti-aging chemical. Methylene Blue can be an MAOI, but maybe at a level much higher than he is taking now (like as much as 0.25mg).
Molecular Weight: 319.85 g/mol
Active MOAI A at: 27nM/L (source) ("...very potent inhibitor of MAO A with a Ki of 27nM...")
Blood Stream Volume: 6L
319.85 * 27 * (10^(-9)) * 6 = 5.18157 × 10-5
So an injection of 52mcg would have some MAOI effect. An ingestion of 60mcg would probably not yield a grand effect on MAO inhibition, seeing how the digestive system would release it at a decreasing rate for 2-4 hours.
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